Detection of TBEV RNA in ticks as a tool for valuation of endemic area wide and sensitivity of TBE surveillance
Makówka, A.; Gut, W.ło.; Rogalska, J.; Michalik, J.; Wodecka, B.; Rymaszewska, A.; Stefanoff, P.ł
Przeglad Epidemiologiczny 63(3): 375-378
2009
ISSN/ISBN: 0033-2100 PMID: 19899594 Document Number: 630001
In this study we present the nested RT-PCR strategy designed for detection of TBEV RNA in ticks Ixodes ricinus. The presented nested RT-PCR method using 2 different primer pairs specific primers for NS5 gene provides specific TBEV cDNA detectable by electroforesis in agarose gel. Of the 177 polls of ticks investigated, TBEV RNA was detected in 14, which accounts for 7.9% of all pools. We confront the PCR results of tested ticks to routine surveillance data. The obtained results showed that the TBEV RNA is detectable in ticks collected in areas in Poland, which are defined as an non-endemic. The nested RT-PCR method can be used as a tool of epidemiological surveillance as well as for screening of occurrence of circulating TBEV.