A technique for determination of free and peptide-bound hydroxyproline in blood serum
Sharaev, P.N.; Sakhabutdinov, E.P.; Lekomtseva, O.I.; Koshikova, S.V.
Klinicheskaia Laboratornaia Diagnostika 1: 7-9
2009
ISSN/ISBN: 0869-2084 PMID: 19253691 Document Number: 629552
A technique has been developed to determine free and peptide-bound hydroxyproline in blood serum. It is based on the principle of chloramine oxidation of hydroxyproline and condensation of oxidation products with p-dimethylaminobenzaldehyde. Data on changes in the serum level of hydroxyproline in children with acute pneumonia and in pregnant women are presented.