The influence of developmental stages and protective additives on cryopreservation of surf clam (Spisula sachalinensis) larvae
Choi, Y.H.; Lee, J.Y.; Chang, Y.J.
Journal of Environmental Biology 29(4): 461-463
2008
ISSN/ISBN: 0254-8704 PMID: 19195381 Document Number: 618255
This study was performed to find out the optimal larval stage and the most desirable protective additives for cryopreservation of surf clam, Spisula sachalinensis larvae. The survival rates of frozen-thawed larvae increased with post developmental stage. The highest value of 96.1 +/- 1.0% was achieved using umbo stage larva as developmental stage and 0.2 M sucrose as protective additive.