Antimicrobial activity of leaf extract of Basilicum polystachyon (L) Moench
Chakraborty, D.; Mandal, S.M.; Chakraborty, J.; Bhattacharyaa, P.K.; Bandyopadhyay, A.; Mitra, A.; Gupta, K.
Indian Journal of Experimental Biology 45(8): 744-748
2007
ISSN/ISBN: 0019-5189 PMID: 17877153 Document Number: 614080
Phenolic extract of leaves of Basilicum polystachyon (L) Moench was tested for in vitro antimicrobial activity against five bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Micrococcus leuteus) and three fungi (Fusarium oxysporum, Aspergillus niger, Helminthosporium oryzae). Efficacy of organic solvents, methanol and ethanol, as agents for extraction was compared with acidic water (2M; HCl). High-pressure liquid chromatographic (HPLC) data showed that acidic extraction (2M; HCl) resulted in higher yield of caffeic acid (0.437 mg g(-1)) and rosmarinic acid (0.919 mg g(-1)). Acidic extract showed high activity against Gram (+) ve bacteria, but was less active against Gram (-) ve bacteria. Amongst the tested fungi, maximum activity was exhibited against Aspergillus niger. This is the first report on the phenolic constituents and bioactivity of B. polystachyon.