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Regulation of osteopontin expression in odontoblasts by upstream stimulatory factor 1

Wu, L.-A.; Wen, L.-Y.; Yang, F.-S.

Zhonghua Kou Qiang Yi Xue Za Zhi 42(11): 663-664

2007

ISSN/ISBN: 1002-0098
PMID: 18307922
Document Number: 612442
To investigate the regulation effects of upstream stimulatory factor 1 (USF1) on osteopontin expression in odontoblasts. Odontoblast MDPC-23 was cultured and stably transfected with PCMV-USF1 or A-USF plasmids. Total RNA was extracted and osteopontin expression examined by semi-quantitative RT-PCR. Gray value of osteopontin was measured and statistic analysis performed. Clones of stable PCMV-USF1 and A-USF plasmids transfection were obtained. Compared with the control, osteopontin was upregulated in PCMV-USF1 transfection group, and downregulated in A-USF transfection group. Upstream stimulatory factor 1 could regulate the osteopontin expression in odontoblasts, which could be blocked partly by A-USF.

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