Expression, subcellular localization and nuclear translocation of transcription factor up stream stimulatory factor-1 in odontoblasts
Wu, L.-A.; Wen, L.-Y.; Yang, F.-S.; Wang, X.-J.; Fang, J.
Zhonghua Kou Qiang Yi Xue Za Zhi 42(9): 559-560
2007
ISSN/ISBN: 1002-0098 PMID: 18070436 Document Number: 610881
To examine the expression and subcellular localization of transcription factor USF1 in odontoblasts and investigate whether nuclear translocation occurs under stimuli. Odontoblasts MDPC-23 were cultured on coverslips and divided into 2 groups. Group 1 received no stimuli, and group 2 was stimulated by nicotine with various concentrations respectively for 1h. Then the mountings of odontoblasts were prepared and immunocytochemical staining was performed with specific USF1 antibody via SABC method. Hela cells were used as positive control. The staining was positive in the cytoplasm of odontoblasts in group 1, but in the nuclei of Hela cells and in 100 mg/L nicotine-stimulated odontoblasts in group 2. There exists USF1 protein in odontoblasts, which locates in the cytoplasm and could translocate into nuclei under the stimulation of nicotine.