Role of polyamines in the function of nuclear transcription factor NF-kappaB in breast cancer cells
Zaletok, S.; Alexandrova, N.; Berdynskykh, N.; Ignatenko, N.; Gogol, S.; Orlovsky, O.; Tregubova, N.; Gerner, E.; Chekhun, V.
Experimental Oncology 26(3): 221-225
2004
ISSN/ISBN: 1812-9269 PMID: 15494691 Document Number: 576039
The aim of the work was the investigation of the influence of polyamines on formation of nuclear transcription factor kappa B (NF-kB) complex with specific DNA-sequences nuclear response elements (NRE), and the study of content of p50 and p65 (NF-kB subunits) in breast tumor cells under polyamines metabolism modulation. Methods: The model experiments in vitro were carried out on cultured MCF-7 cells, in vivo experiments - on experimental Ca755 and Walker-256 tumors using surface plasmon resonance (SPR) and Western-blotting analysis. Results: The data have demonstrated that polyamines falicitated p50 - NRE binding. Under conditions of polyamine depletion, an increase of content of both p50 and p65 subunits of NF-kB in the nucleus has been registered (especially that of p50 subunits). Upon addition of alpha-DFMO + putrescine to the culture medium of MCF-7 cells, the decrease of the levels of p50 and p65 proteins in the nuclei has been observed. Conclusion: Polyamines influence the activity of NF-kB transcription factor in breast tumor cells.