Differential expression of osteopontin and bax, bcl-2 by fluoride
Xu, H.; Zhang, J-min.; Zhang, X-yun.; Li, G-sheng.
Zhonghua Yu Fang Yi Xue Za Zhi 39(2): 107-110
2005
ISSN/ISBN: 0253-9624 PMID: 15842830 Document Number: 593570
Objective To study the differential expression of bax, bcl-2 and osteopontin by fluoride in the renal tubular cells in vitro. Methods The renal tubular cells were cultured and exposed to sodium fluoride (NaF) in 1, 5, 7.5, 12.5 mg F-/L level. The transcription level of bax, bcl-2 and osteopontin were investigated by reverse transcription - polymerase chain reaction (RT-PCR). Results The expression of bax mRNA in 7.5 and 12.5 mgF(-)/L groups (optical absorption ratio value was 2.37 +/- 0.18 and 2.64 +/- 0.19 respectively) was significantly increased (P < 0.01). On the contrary, the level of bcl-2 obviously decreased (5 mg F-/L group optical absorption ratio value, 0.80 +/- 0.22, P < 0.05; 7.5 mg F-/L group optical absorption ratio value 0.71 +/- 0.22, P < 0.01). The expression mRNA of osteopontin was significantly increased when cells were exposed to fluoride at 7.5 mg F-/L (optical absorption ratio value 2.01 +/- 0.40 P < 0.01), in that group the tubular cell apoptotic trend was obvious. Conclusion NaF might induce tubular cell apoptosis via activation of bax expression and bcl-2 suppression. Osteopontin might protect the tubule against apoptosis in a lower fluoride level, but the function should be decreased in higher fluoride level.