The study on the chromosome aneuploidy in human lung cancer
Gao, S.-g.; Dong, X.-y.; Wang, L.-j.; He, J.
Zhonghua Yi Xue Za Zhi 87(24): 1701-1703
2007
ISSN/ISBN: 0376-2491 PMID: 17825154 Document Number: 611445
To study the feasibility of interphase fluorescence in situ hybridization (FISH) in detection of chromosomal aneuploidy in touch preparations of lung carcinoma and its clinic significance. We examined 46 touch preparations of lung carcinomas by FISH with DNA specific for chromosomes 7, 8, 9 and 12 to detect chromosomal aneuploidy. Aneuploidy, mainly featured in gains of chromosomes 7, 8 and 12 and loss of chromosome 9 were observed in all of the touch preparations of the 46 cases with lung cancer. The rates of chromosomal aneuploidy detected by FISH were 67.4% (31/46), 60.9% (28/46), 28.3% (13/46) and 56.5% (26/31) respectively. Interphase FISH is feasible in detecting aneuploidy in touch preparations of lung cancer. These alterations may be applied to early diagnosis and predicting prognosis of lung cancer.