Parallel analysis of c-Fos protein and interleukin-2 expression in hypothalamic cells under different influence
Barabanova, S.V.; Artiukhina, Z.E.; Ovchinnikova, K.T.; Abramova, T.V.; Kazakova, T.B.; Khavinson, V.K.; Malinin, V.V.; Korneva, E.A.
Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova 93(2): 150-160
2007
ISSN/ISBN: 0869-8139 PMID: 17461018 Document Number: 607990
The objective of this work was to perform a parallel analysis of activation of the rat anterior hypothalamus cells as judged by c-Fos protein expression, and of the expression of interleukin-2 (IL-2) under different influences, i. e., mild stress (handling) and adaptation to it, and intranasal administration of saline and the peptides Vilon (Lys-Glu) and Epithalon (Ala-Glu-Asp-Gly). Changes in the counts of cells positive for c-Fos- and IL-2 proteins were studied in structures of the lateral (LHA) area, anterior (AHN), supraoptic (SO) and paraventricular (PVH) nuclei of Wistar rat hypothalamus. Quantity of the interleukin-2-positive and c-Fos-positive cells was calculated. The findings were: a negative correlation between the activation of cells and the amount of IL-2 in the cells in the hypothalamic structures under study, and the specific patterns of changes in the counts of cells positive for c-Fos and IL-2 under stress and adaptation to stress.