Interleukin 1 beta and tumour necrosis factor alpha stimulate the release of gonadotropin-releasing hormone and interleukin 6 by primary cultured rat hypothalamic cells
Yamaguchi, M.; Yoshimoto, Y.; Komura, H.; Koike, K.; Matsuzaki, N.; Hirota, K.; Miyake, A.; Tanizawa, O.
Acta Endocrinologica 123(4): 476-480
1990
ISSN/ISBN: 0001-5598 PMID: 2239094 Document Number: 364462
The abilities of recombinant human interleukin 1.beta. and tumour necrosis factor .alpha. to induce release of GnRH and interleukin 6 from primary culture of rat hypothalamic cells were examined. The effect of estradiol on the release of interleukin 6 by these cells was also tested. Both interleukin 1.beta. and tumour necrosis factor .alpha. caused significant stimulation of GnRH secretion from the hypothalamic cells within 5 min. The hypothalamic cells secreted interleukin 6 spontaneously, and their secretion over 24 h was stimulated dose-dependently by interleukin 1.beta., tumour necrosis factor .alpha. and estradiol. These results suggest that interleukin 1.beta. and tumour necrosis factor .alpha. stimulate the secretions of GnRH and interleukin 6 in the hypothalamus, and that these cytokines may be involved in the mechanism of GnRH secretion in the hypothalamus.