Cultured skin cells interaction with polylactide surface coated by different collagen structures
Shved, I.A.; Kukhareva, L.V.; Zorin, I.M.; Blinova, M.I.; Bilibin, A.I.; Pinaev, G.P.
Tsitologiia 49(1): 32-39
2007
ISSN/ISBN: 0041-3771 PMID: 17432605 Document Number: 606884
The purpose of this work was an optimization of polylactide film surfaces designed for human keratinocytes cultivation. The polylactide films were coated by collagen 1. The experiments showed that uniform covering of polymer surface by collagen, and formation of different collagen structures depend on the mode of the protein application. The differences in collagen distribution on the polymer surface influened the keratinocytes growth in culture. Analysis of keratinocytes alignment, as well as cytoskeleton organization demonstrated that fibrillar collagen promoted more even keratinocytes distribution in comparison with the distribution on molecular collagen.