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Time course changes in cytoskeletal structures of cultured endothelial cells exposed to shear stress

Ookawa, K.; Sato, M.; Ohshima, N.

Frontiers of Medical and Biological Engineering the International Journal of the Japan Society of Medical Electronics and Biological Engineering 5(2): 121-125

1993

ISSN/ISBN: 0921-3775
PMID: 8241028
Document Number: 405262
Changes in patterns of microfilament distribution were analyzed using cultured porcine aortic endothelial cells under shear flow. Within the elongated endothelial cells after shear flow exposure (20 dyne/cm-2, 0-24 h), F-actin filaments were mainly observed as stress fibers. After 24 h exposure, the fluorescence intensity of rhodamine-phalloidin-stained cells was enhanced, indicating the increase in F-actin content. It was suggested that not only F-actin distribution but also the content may be affected by shear stress at the early stage of exposure.

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