Studies on bioassay-guided anti-inflammatory fraction in bark of Albizia julibrissin combined determination with LC-MS-MS
Qiao, S.-Y.; Yu, D.-H.; Guo, J.-F.; Zhao, Y.-M.
Zhongguo Zhong Yao Za Zhi 32(19): 2021-2025
2007
ISSN/ISBN: 1001-5302 PMID: 18161296 Document Number: 606443
To search the anti-inflammatory fraction of Albizia julibrissin. Inflammatory model of Kunming mice ear edema induced by croton oil and determination combined with the LC-MS-MS-guided fractionation and isolation were used. The n-butanol fraction (AJ-B) obtained from the ethanolic extract of the Cortex albiziae was the major active fraction. The lignan glycosides fraction (AJ-B-1), which was further isolated from AJ-B, showed significant anti-inflammatory activity and exhibited dose-dependent relationship in the dose of 5 to 20 mg x kg(-1). The method of bioassay-guided fractionation and isolation combined with the LC-MS-MS determination may be of benefit to the logical studies on the bioactive fractions or constituents of traditional Chinese materia medica.