Small interfering RNA (siRNA) inhibited human liver cancer cell line SMMC7721 proliferation and tumorigenesis
Dong-Dong, L.
Hepato-Gastroenterology 54(78): 1731-1735
2007
ISSN/ISBN: 0172-6390 PMID: 18019706 Document Number: 605471
Background/Aims: The purpose of this study is to understand how AY.R1C2 small interfering RNA (siRNA) influences human liver cancer cell line SMMC7721 proliferation and tumorigenesis in vitro and in vivo.Methodology: We constructed AKRIC2 small interfering RNA (siRNA) expression vector pGenesil-1/AKR1C2 RNAi and then transfected it into liver cancer cell line SMMC7721. We analyzed SMMC7721 cells proliferation by MTT method, cell cycle distribution determined by measuring the cellular DNA content using flow cytometry, colonyformation efficiency, tumorigenesis in vivo.Results: SMMC7721 cells stably transfected with pGenesil-1/AKR1C2 RNAi showed a significant increase in cells of the GO/G1 phase and a significant decrease in cells of the S phase and G2/M phase than in mock cells transfected with pGenesil-1. AKR1C2 siRNA significantly inhibited the cell proliferation (p<0.01) and resulted in a lower colony-formation efficiency in soft agar P<0.01), and decreased tumor formation ability in vivo.Conclusions: AKR1C2 RNAi could lead to alteration of SMMC7721 cell cycle and inhibit turmorigenesis in vivo and in vitro, which strongly suggests that AKR1C2 siRNA might play a critical role in blocking hepatocarcinogenesis.