Top Abstract Purchase Related
Home
>
Node
>
Subnode

Inhibition of cell proliferation and C-myc cancer protein expression in human colon adenocarcinoma cell line HT29 with VIP-131I-ASON

Ou, X.; Tan, T.; Li, Y.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 23(5): 1096-1100

2006

ISSN/ISBN: 1001-5515
PMID: 17121362
Document Number: 600521
A 15-mer phosphorothioate antisense oligonucleotide (ASON) complementary to the translation start region of the C-myc oncogene mRNA was labeled with 131I or 125I and the labelled compound was linked to the vasoactive intestinal peptide (VIP) to be bound covalently to a polylysine chain so as to deliver oligonucleotide into tumor cells. The effect of the VIP as carrier on cell uptake of ASON in tissue culture was evaluated in a human colon adenocarcinoma HT29 cell line. The efficacy of VIP-131-ASON on cell growth was evaluated using the MTT assay. Expression of c-myc-encoded protein was measured by flow cytometry. Sense and nosense control Oligonucleotides with VIP carrier were used as control. The results showed that VIP competed effectively with VIP-125I-ASON to bind the HT29 cells. Cell uptake was increased 3-4 fold using the VIP carrier compared to the same dosage of naked DNA. HT29 cells treated with VIP-131I-ASON complexes exhibited 4-fold lower proliferation than those treated with 13I-ASON and 6-fold lower proliferation than those treated with radioiodinated Sense and nosense DNA. Cancer protein expression of HT29 cells treated with VIP-131I-ASON was decreased 2-fold compare with that in 131I-ASON treated cell. The use of a VIP carrier greatly increased 131I-ASON cellular uptake and inhibition of cell proliferation and C-myc cancer protein expressing in HT29 cell by radioiodinated antisense Oligonucleotides.

Document emailed within 1 workday
Secure & encrypted payments

Jiang, W.G.; Hiscox, S. 1996: Cytokine regulation of ezrin expression in the human colon cancer cell line HT29 Anticancer Research 16(2): 861-865
Hsieh, M.L.; Charlesworth, M.C.; Goodmanson, M.; Zhang, S.; Seay, T.; Klee, G.G.; Tindall, D.J.; Young, C.Y. 1997: Expression of human prostate-specific glandular kallikrein protein (hK2) in the breast cancer cell line T47-D Cancer Research 57(13): 2651-2656
Poulin, R.; Labrie, F. 1986: Stimulation of cell proliferation and estrogenic response by adrenal C19-delta 5-steroids in the ZR-75-1 human breast cancer cell line Cancer Research 46(10): 4933-4937
Chaouki, W.; Leger, D.Y.; Liagre, B.; Cherrah, Y.; Beneytout, J.-L.; Hmamouchi, M. 2009: Roots of Daphne gnidium L. inhibit cell proliferation and induce apoptosis in the human breast cancer cell line MCF-7 Die Pharmazie 64(8): 542-546
Lu, W.-q.; Wang, F.; Liu, H.-y. 2013: Influence of oxaliplatin combined with LCP on proliferation and apoptosis of colon cancer cell line HT29 Zhonghua Wei Chang Wai Ke Za Zhi 16(1): 84-88
Battu, S.; Rigaud, M.; Beneytout, J.L. 1998: Resistance to apoptosis and cyclooxygenase-2 expression in a human adenocarcinoma cell line HT29 CL.19A Anticancer Research 18(5a): 3579-3583
Shoyab, M.; Mcdonald, V.L.; Dick, K.; Modrell, B.; Malik, N.; Plowman, G.D. 1991: Amphiregulin-associated protein: complete amino acid sequence of a protein produced by the 12-0-tetradecanoylphorbol-13-acetate-treated human breast adenocarcinoma cell line MCF-7 Biochemical and Biophysical Research Communications 179(1): 572-578
Uberall, F.; Maly, K.; Egle, A.; Doppler, W.; Hofmann, J.; Grunicke, H.H. 1991: Inhibition of cell proliferation, protein kinase C, and phorbol ester-induced fos expression by the dihydropyridine derivative B859-35 Cancer Research 51(21): 5821-5825
Dahiya, R.; Lesuffleur, T.; Kwak, K.S.; Byrd, J.C.; Barbat, A.; Zweibaum, A.; Kim, Y.S. 1992: Expression and characterization of mucins associated with the resistance to methotrexate of human colonic adenocarcinoma cell line HT29 Cancer Research 52(17): 4655-4662
Kikuchi, Y.; Kizawa, I.; Oomori, K.; Miyauchi, M.; Kita, T.; Sugita, M.; Tenjin, Y.; Kato, K. 1987: Establishment of a human ovarian cancer cell line capable of forming ascites in nude mice and effects of tranexamic acid on cell proliferation and ascites formation Cancer Research 47(2): 592-596
Hu, Y.-L.; Wang, X.-B.; Chen, D.-D.; Guo, X.-J.; Yang, Q.-J.; Dong, L.-H.; Cheng, L. 2016: Germanicol induces selective growth inhibitory effects in human colon HCT-116 and HT29 cancer cells through induction of apoptosis, cell cycle arrest and inhibition of cell migration Journal of Buon: Official Journal of the Balkan Union of Oncology 21(3): 626-632
Albert, J.L.; Sundstrom, S.A.; Lyttle, C.R. 1990: Estrogen regulation of placental alkaline phosphatase gene expression in a human endometrial adenocarcinoma cell line Cancer Research 50(11): 3306-3310
Robbins, P.F.; Kantor, J.A.; Salgaller, M.; Hand, P.H.; Fernsten, P.D.; Schlom, J. 1991: Transduction and expression of the human carcinoembryonic antigen gene in a murine colon carcinoma cell line Cancer Research 51(14): 3657-3662
Targosz, A.; Pierzchalski, P.; Krawiec, A.; Szczyrk, U.; Brzozowski, T.; Konturek, S.J.; Pawlik, W.W. 2006: Helicobacter pylori inhibits expression of heat shock protein 70 (HSP70) in human epithelial cell line. Importance of Cag A protein Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society 57(2): 265-278
Liao, Y-de.; Long, Q-hong.; Zhou, S.; Zhao, J-ping.; Huang, Q.; Fu, X-ning. 2005: Expression of PKB protein in human squamous-cell carcinoma and adenocarcinoma of lung Zhonghua Zhong Liu Za Zhi 27(3): 156-159
Ikegami, Y.; Yano, S.; Nakao, K. 1996: Effects of the new selective protein kinase C inhibitor 4'-N-benzoyl staurosporine on cell cycle distribution and growth inhibition in human small cell lung cancer cells Arzneimittel-Forschung 46(2): 201-204
Wang, Y.; Tong, T.; Guo, S. 1999: Alterations of cell apoptosis and proliferation during transformation of a human bronchial epithelial cell line Zhonghua Zhong Liu Za Zhi 21(3): 168-170
Ballen, K.K.; Ritchie, A.J.; Murphy, C.; Handin, R.I.; Ewenstein, B.M. 1996: Expression and activation of protein kinase C isoforms in a human megakaryocytic cell line Experimental Hematology 24(13): 1501-1508
Berman, E.; Rubin, B.Y.; Broxmeyer, H.E. 1986: Regulation of the proliferation of the established human monoblast cell line, U937, at the single cell level Cancer Research 46(7): 3309-3312
Li, H.F.; Huang, L.F.; Chen, L.H. 2019: Chitooligosaccharides inhibit A549 lung cancer cell line proliferation by regulating cell autophagy Journal of Biological Regulators and Homeostatic Agents 33(5): 1527-1532