Determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products by HPLC
Liu, F.; Yu, X.-H.; Li, F.; Tan, Y.-Y.; Qiao, Y.-J.
Zhongguo Zhong Yao Za Zhi 31(14): 1160-1162
2006
ISSN/ISBN: 1001-5302 PMID: 17048584 Document Number: 604991
To establish an HPLC method for the determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products namely mesaconitine, aconitine and hypaconitine. A Zorbax Eclipse XDB C18 column was used, and ammonium acetate solution-acetonitrile as the mobile phase, with a flow rate of 1.0 mL x min(-1) and a detection wavelength of 230 nm. Mesaconitine, aconitine and hypaconitine, were separated, and the calibration curves of them were in good linearity over the range of 0.035 7-1.784 microg (r = 0.9999), 0.0126-0.632 microg (r = 0.9997) and 0.0334-1.672 microg (r = 0.9997) respectively. This method is simple and accurate. It can be used in the identification and quality control of A. carmichaeli.