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Studies on swinepox virus: fluorescence and light microscopy of infected cell cultures

Garg, S.K.; Meyer, R.C.

Research in Veterinary Science 14(2): 216-219

1973

ISSN/ISBN: 0034-5288
PMID: 4141785
Document Number: 60495
Swinepox virus was cultivated in PK-15 and primary pig kidney cells, and the developing nuclear and cytoplasmic inclusions were studied by light and fluorescence microscopy employing acridine orange (AO) and specific fluorescent antibody (FA). Both nuclear and cytoplasmic inclusions were evident within 24-48 hours after inoculaton. The cytoplasmic inclusions gave a positive FA reaction and a strong greenish fluorescence when stained with AO. The nuclear inclusions appeared as round to oval vacuoles, gave a negative FA reaction, and only a faint greenish fluorescence when stained with AO. The formation of the nuclear inclusion appears to be an indirect result of the viral infection. At no time could viral antigen be demonstrated in either nucleus or nuclear inclusion.

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