Inhibition of EBV-encoded LMP-1 by DNA-based RNA interference affects metastatic ability of nasopharyngeal carcinoma cells
Li, G.; Li, X.-P.; Liu, X.; Peng, Y.; Lin, M.C.
Zhonghua Zhong Liu Za Zhi 28(10): 724-727
2006
ISSN/ISBN: 0253-3766 PMID: 17366780 Document Number: 603722
To evaluate the application of DNA vector-based RNA interference for silencing LMP-1 expression in EBV-positive nasopharyngeal cancer (NPC) C611 cells and analyze the inhibiting effects of LMP-1 silencing on metastatic property of NPC cells. DNA vectors with LMP-1 shRNA template were transfected into C611 cells. After G418 selection, a LMP-1 silenced sub-clone named C611L was isolated. Effect of LMP-1 inhibition on its metastatic properties was evaluated by cell adhesion, invasion and wound healing assays. shRNA vector mediated stable RNAi against LMP-1 in C611 cells. Loss of LMP-1 significantly altered cell motility, substratum adhesion and transmembrane invasion ability. Stable suppression of LMP-1 induced by shRNA vector can inhibit metastatic properties of NPC cells.