Suppression of c-myc expression by interference RNA in HepG2 hepatocellular carcinoma cells
Xu, Y.; Wang, Y-Hua.; Gao, J-Dong.; Ye, J.; Zhu, H-Xia.; Xu, N-Zhi.; Wang, X-Yu.; Sun, Z-Tang.
Zhonghua Zhong Liu Za Zhi 26(8): 458-460
2004
ISSN/ISBN: 0253-3766 PMID: 15555332 Document Number: 570633
Objective To study the inhibitory effect of RNA interference (RNAi) on c-myc expression in hepatocellular carcinoma cell line, HepG2. Methods Expression vector of c-myc gene-targeting small interference RNA (siRNA) was constructed (psilencer-c-myc) and transfected into HepG2 cells by lipofectamine, and the unloaded vector was used as control (mock). The expression of c-myc mRNA and protein was identified by quantitive PCR and Western blot. Apoptosis of the transfected cells was examined by flow cytometry and immunofluorescent microscopy. Results After HepG2 cells were transfected with psilencer-c-myc, the expression of c-myc mRNA and protein was suppressed with an inhibition rate of 67% compared with the mock-transfected cells. Apoptosis was identified in the transfected HepG2 cells. Conclusion The expression of c-myc at transcriptional and translational levels in HepG2 cells transfected with siRNA is markedly inhibited, which may be associated with the induction of apoptosis.