Apoptosis of human malignant lymphoid NAMALWA cells induced by resveratrol and quercetin
Fil'chenkov, A.A.; Zavelevich, M.P.; Khranovskaia, N.N.; Mykhaĭlenko, V.M.
Ukrains'kyi Biokhimichnyi Zhurnal 78(4): 112-119
2006
ISSN/ISBN: 0372-3909 PMID: 17236628 Document Number: 600807
The effects of naturally-occuring polyphenols, resveratrol and quercetin, on cell viability and apoptosis were studied in Namalwa B-cell lymphoma line. Apoptotic cells were identified using DNA flow cytometric analysis and 1H NMR spectroscopy. The effects of the agents on the cell cycle kinetics and activation of caspase-3 were examined. Both resveratrol and quercetin induced apoptosis in Namalwa cells as demonstrated by the increased number of hypodiploid cells, elevated level of mobile lipid domains and caspase-3 activation. Treatment with 40 microM of resveratrol for 48 h resulted in time-dependent cell-cycle arrest at G0/G1. In contrast, upon quercetin treatment Namalwa cells accumulated in G2/M. Obtained results suggest that resveratrol and quercetin induced caspase-dependent apoptosis in human malignant lymphoid cells in vitro. These findings provide a rationale for further studies of in vivo effects of those polyphenols.