Mechanisms of capsaicin-induced apoptosis of human melanoma A375-S2 cells
Gong, X-feng.; Wang, M-wei.; Ikejima, T.
Zhonghua Zhong Liu Za Zhi 27(7): 401-403
2005
ISSN/ISBN: 0253-3766 PMID: 16188123 Document Number: 591974
Objective To study the mechanisms of capsaicin-induced apoptosis of human melanoma A375-S2 cells. Methods MTT assay, fluorescence microscopy, DNA agarose gel electrophoresis, flow cytometry and Western blot analysis were carried out to assess the morphological and biochemical changes of A375-S2 cells after capsaicin treatment. Results Capsaicin induced A375-S2 cell death in a time- and dose-dependent manner. Sub-diploid peak was seen at 24 h after 250 mu mol/L capsaicin treatment, and apoptotic bodies and DNA ladder were observed at 36 h after capsaicin treatment. The expression of inhibitor of caspase activated DNase(ICAD)was reduced with the lapse of time. Conclusion Capsaicin induces A375-S2 cell apoptosis and dowry-regulation of ICAD contributes to this process.