Apoptosis induced by proteasome inhibition in human myoblast cultures
Sassone, J.; Ciammola, A.; Tiloca, C.; Glionna, M.; Meola, G.; Mancinelli, E.; Silani, V.
European Journal of Histochemistry Ejh 50(2): 109-118
2006
ISSN/ISBN: 1121-760X PMID: 16864121 Document Number: 597680
Dysfunction of the ubiquitin-proteasome system has recently been implicated in the pathogenesis of some untreatable myodegenerative diseases characterized by the formation of ubiquitinated inclusions in skeletal muscles. We have developed an in vitro model of proteasomal dysfunction by applying inhibitors of the proteasome to primary adult human skeletal muscle cultures. Our data show that proteasome inhibition causes both cytoplasmic accumulation of ubiquitinated inclusions and apoptotic death, the latter through accumulation of active caspase-3.