Expression of mineral-associated factors in the mineral stage tooth germ of mice
Pan, K.-q.; Yang, P.-s.; Li, S.
Shanghai Kou Qiang Yi Xue 15(4): 383-387
2006
ISSN/ISBN: 1006-7248 PMID: 16955163 Document Number: 595548
To study the distribution and expression of core binding factor alpha 1 (Cbf alpha1), bone sialoprotein (BSP), osteopontin (OPN), osteocalcin (OC) and alkaline phosphatase (ALP) in mineral stage tooth germ of mice, so as to understand their roles in the development of the mineral stage tooth germ. Thirty BALB/c mice in different days were killed, and their bilateral mandibular first molar germs with surrounding alveolar bone were taken out, then the tissues were fixed with 4% paraformaldehyde at 4 degrees C overnight, dehydrated, embedded in paraffin and serially sectioned at 5 microm. Immunohistochemical assay was adopted to determine the tissue distribution and cellular localization of Cbf alpha1, OPN, BSP, ALP and OC in the mineral stage tooth germ by these sections of BALB/c mice. There were Cbf alpha1, BSP and OPN expressing in the alveolar bone, and Cbf alpha1, ALP, OC in predentin, while in other tissues such as dental follicle, pulp cells, stratum intermedium, stellate reticulum, only ALP and OC were detected, but none of them were observed in the dentin. The formation mechanisms of hard tooth tissues are different. They are controlled by different factors. Cbf alpha1, ALP and OC are involved in the early formation of dentin, while Cbf alpha1, OPN and BSP involved in the early formation of alveolar bone, ALP involved in the stratum intermedium. They all play an important role in the start of mineralization.