Growth factor NUN increased AKR1C2 expression by activated CDK2 related RB signal transduction pathway in human liver cancer cell line
Lu, D-Dong.
Hepato-Gastroenterology 52(62): 567-570
2005
ISSN/ISBN: 0172-6390 PMID: 15816479 Document Number: 594661
Background/Aims: The investigation is to understand whether polypeptide growth factor NUN can enhance AKR1C2 transcriptional expression human SMMC7721 liver cancer cell line or not. Methodology: SMMC7721 cell was starved in serum-free medium overnight before NUN treatment, and cells were then-incubated with NUN for different times and dosage-. These cells lysates were prepared to examine AKR1C2 expression by Western blot. Total RNA was extracted from these cells to analyze the change of AKR1C2 mRNA by Northern blot. Results: NUN increased expression of AKR1C2 in SAMC7721 liver cancer cell in a time- and- dosagedependent manner and this increase resulted from, up-regulation of AKR1C2 mRNA expression: The inhibitor of CDK2 inhibited the up-regulation of AKR1C2 by NUN. Conclusions: These results showed that NUN can, enhance AKR1C2 transcription by activated CDK2 related RB signaling pathway, leading to increased AKR1C2 expression in SMMC7721 liver cancer cell.