Multiple use of a signal transduction pathway in tumor cell invasion
Tímár, J.; Rásó, E.; Fazakas, Z.S.; Silletti, S.; Raz, A.; Honn, K.V.
Anticancer Research 16(6a): 3299-3306
1996
ISSN/ISBN: 0250-7005 PMID: 9042304 Document Number: 462580
Recent reports have indicated that a poorly characterized signaling pathway involving 12-lipoxygenase and PKC is involved in several steps of the metastatic cascade such as tumor cell adhesion to matrix, lysosomal enzyme release and migration. We investigated here the upstream elements of this signalling pathway looking for receptors which it might be coupled to. We described tumor cell aIIb-beta-3-like integrin and the AMF receptor, gp78, as two transmembrane receptors which utilize the 12-lipoxygenase/PKC signaling. Furthermore, we compared the in vivo expression of the signal components of the "autocrine motility cycle" (gp78, 12-LOX and PKC-alpha - Timar et al 1993) in human prostate carcinoma cell lines (PC3 and DU145) with different invasive potential. We report here that all three components of this cycle are overexpressed three fold in vivo in the more aggressive tumor, suggesting that this signalling pathway might regulate the invasive phenotype - at least in the studied tumor cells.