Molecular identity of double-stranded RNA-elements of viral nature isolated from the sugar beet
Mel'nichuk, M.D.; Spiridonov, V.G.
Mikrobiolohichnyi Zhurnal 67(2): 55-62
2005
ISSN/ISBN: 0201-8462 PMID: 16018217 Document Number: 593542
Hybridization analysis of the double-strand RNA-elements isolated from the sugar beet of the Kyiv, Vinnytsya and Poltava Regions was carried out. A positive signal has been obtained with the dsRNA fragment, 1.9 kbp long, which codes the viral RNA- dependent RNA-polymerase having high level of homology with the analogous enzyme of mycoviruses from Partitiviridae family. Molecular identity of these dsRNA-elements in the sugar beet samples from the Vinnytsya and Poltava regions has been shown The RT-PCR test system has been developed for detecting the nondescribed mycovirus which is proposed to be called Helicobasidium purpureum partitivirus.