Genetic diversity and molecular authentication of wild populations of Dendrobium officinale by RAPD
Ding, G.; Ding, X.-y.; Shen, J.; Tang, F.; Liu, D.-y.; He, J.; Li, X.-x.; Chu, B.-h.
Yao Xue Xue Bao 40(11): 1028-1032
2005
ISSN/ISBN: 0513-4870 PMID: 16499089 Document Number: 590631
Genetic diversity, relationship and molecular authentication of total 8 wild populations of Dendrobium officinale were investigated using RAPD markers. 10 random decamer primers were screened for Random Amplified Polymophic DNA (RAPD) fragments. A DNA molecular dendrogram was established based on cluster analysis by UPGMA (unweighted pair-group method with arithmetic average), and the relationship of the wild populations were analyzed, and all the wild populations were authenticated. A total of 439 loci with an average of 43.9 loci per primer and 54.9 loci per population were amplified from 8 wild populations by 10 effective primers. In the total 104 amplified bands, 95 were polymorphic, corresponding to 91.35% genetic polymorphism. The genetic distances were 0. 590 to 0. 727, with an average of 0. 686. Distinct genetic differences and extensive genetic diversity were presented among the wild populations. RAPD markers were an informative and useful tool for the genetic diversity, evaluation and authentication of wild populations of Dendrobium officinale. Primer S412 could be used to authenticate 8 wild populations completely.