Use of RAPD-PCR fingerprinting to detect genetic diversity of soil Streptomyces isolates
Malkawi, H.I.; Saadoun, I.; Moumani, F.A.; Meqdam, M.M.
New Microbiologica 22(1): 53-58
1999
ISSN/ISBN: 1121-7138 PMID: 10190117 Document Number: 503590
Random amplified polymorphic DNA (RAPD) was used for identification and assessment of genetic diversity between isolates of Streptomyces from soil. Genomic DNA from 18 Streptomyces isolates and 2 reference strains were amplified using four different 10-mer primers. Different DNA fingerprinting patterns were obtained for all the isolates. Electrophoretic and cluster analysis of the amplification products showed polymorphism among the isolates and none of them was identical to the reference strains although there were some common amplification bands. Two highly divergent groups were determined among the isolates. The results indicate that RAPD is an efficient method for discriminating and studying genetic diversity of Streptomyces isolates.