Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas
Luczyński, Włodzimierz.; Kovalchuk, O.; Krawczuk-Rybak, M.; Mitura-Lesiuk, Młgorzata.; Malinowska, I.; Chyczewski, L.; Kowalczyk, J.; Matysiak, Mł.
Folia Histochemica et Cytobiologica 43(3): 169-171
2005
ISSN/ISBN: 0239-8508 PMID: 16201318 Document Number: 590512
The complex interactions between cancer and host cells are far from being fully elucidated. Assessment of Th-1/Th-2/Th-3/Tr-1 balance is an interesting approach to explain immunological disturbances in lymphomas. The aim of our study was to assess mRNA for pro- and anti-inflammatory cytokines in T-cells in 20 children with Hodgkin- and non-Hodgkin lymphomas. CD4(+) and CD8(+) cells were isolated from whole peripheral blood and four different cytokine mRNA levels (IFN-gamma, IL-10, IL-4, TGF-beta) were determined by real-time PCR technique. Comparing to the control group, we found lower expression of mRNA for IFN-gamma in CD4(+) cells at the time of lymphoma diagnosis. It may be one of the pathogenetic mechanisms of impaired immunity in these patients.