Development of proteome two-dimensional electrophoresis technology
Du, P.; Feng, W.-h.; Guo, J.-s.
Wei Sheng Yan Jiu 34(2): 237-240
2005
ISSN/ISBN: 1000-8020 PMID: 15952674 Document Number: 590398
Two-dimensional electrophoresis (2-DE) is the core technology of proteome study. This paper mainly introduces the 2-DE principle, sample preparation and usual methods to improve its resolving power. Furthermore, isoelectric focusing, SDS-PAGE electrophoresis and 2-DE staining methods are also reviewed.
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