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2-dimensional electrophoresis of cerebrospinal fluid in various neurological patients

Wiederkehr, F.; Vonderschmitt, D.J.

Schweizerische Medizinische Wochenschrift 115(11): 368-373

1985

ISSN/ISBN: 0036-7672
PMID: 3920755
Document Number: 249053
At present 2-dimensional (2D) electrophoresis is the best method of resolution of separating complex protein mixtures, and this method has been successfully adapted for resolving CSF proteins. CSF from over 200 patients with a wide spectrum of neurological diseases were analyzed by 2D electrophoresis in addition to agarose electrophoresis. After a 2D run several hundred fractions of proteins were obtained. Oligoclonal zone patterns not previously described in the light chain region of the Ig in CSF samples were demonstrated. All patients with definite diagnosis of multiple sclerosis (MS), and also other diseases such as neurosyphilis, myelitis, herpes zoster, etc. showed particular oligoclonal zone patterns. This experience indicates that the detection of oligoclonal zones in the region of the Ig-light chain is of major diagnostic significance. Moreover, the region of the so-called CSF-specific protein deserves special attention.

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