Application of two-dimensional electrophoresis and mass spectrometry in the research of testicular proteins
Zhu, Y.-F.; Ma, X.; Zhou, Z.-M.; Sha, J.-H.
Zhonghua Nan Ke Xue 9(2): 85-89
2003
ISSN/ISBN: 1009-3591 PMID: 12749122 Document Number: 556629
To evaluate the application of two-dimensional electrophoresis and mass spectrometry in the research of proteins expressed in testis. Protein from adult ICR mouse testes was extracted by two-dimensional electrophoresis. Two protein spots were cut from the gel, then the proteins were digested in-gel by enzyme and the generated peptides were measured by matrix assisted laser desorption/ionization-time of flight mass spectrometry. The proteins were identified through database searching. Two spots in Commasie Brilliant Blue-stained gel were identified as serum albumin and protein disulfide isomerase by database searching. This rapid high resolution and efficient method is a very powerful way to analyze testicular proteins.