Culture of adult mouse neurons
Eide, L.; McMurray, C.T.
Biotechniques 38(1): 99-104
2005
ISSN/ISBN: 0736-6205 PMID: 15679091 Document Number: 587618
Primary neuronal cells used to model physiology are generally limited to embryonic tissue. However embryonic tissue is not optimal as a model for age-related changes in physiology or late-onset disease. Successful culturing of neurons from adult animals, however has been historically difficult, if not impossible. Here, we report methodology for routine and reliable cultivation of healthy striatal neurons from adult mice. The new methodology is cost-effective and improves the speed and simplicity if neuronal isolation.