Differentiation of dofaminergic neurons from the human embryonal nerve cells in culture
Tsymbaliuk, V.I.; Vasyl'ieva, I.H.; Oleksenko, N.P.; Chopyk, N.H.; Tsiubko, O.I.; Halanta, O.S.
Tsitologiia i Genetika 43(3): 36-41
2009
ISSN/ISBN: 0564-3783 PMID: 19938635 Document Number: 631322
The aim of our experiments was to determine the dofaminergic differentiation potential of human embryonic nerve tissue in culture to obtain cell material for neurotransplantation enriched with determinative dofaminergic cells precursors. During the period of culturing their amount in the experimental samples reliable increased from 5.6 +/- 4.3% dofaminergic neurons cells in the field of vision to 23.5 +/- 2.6%. At the same time in the control samples their concentration was 8.1 +/- 6.5% cells in the field of vision. Dofaminergic differentiation was confirmed by expression analyses of genes associated with this type of neuronal differentiation. We have demonstrated that during the period of culture expression of early transcription factors--Nurr1 and Lmx1b disappears while TH expression level increases. So, using specific culture condition the cell material for neurotransplantation enriched by dofaminergic neuron precursors can be obtained.