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Differentiation of neurons of the cingulate region in explant culture

Tapp, R.; Grosse, G.

Zeitschrift für Mikroskopisch-Anatomische Forschung 93(5): 931-950

1979

ISSN/ISBN: 0044-3107
PMID: 545935
Document Number: 142736
Structural changes in explant-cultures of the cingulate region from the brains of 18 day old rat embryos were investigated during several cultivation periods by neurohistological light microscopical methods. The differentiation and the growth of neurons were observed in cultures stained by the method of Kluever-Barrera, and cut perpendicular to the coverslips during the period from the 1st up to the 20th day in vitro. As parameters for the growth of the cells the density of neurons and glial cells per culture area were estimated and the diameters of nuclei and perikarya were measured at different stages of culture.

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