DNA, proteins and lipids distribution in the cells of olfactory bulbs of rats of various ages
Vekshin, N.L.; Me'lnikova, E.V.; Riabokon', E.N.; Shishmakov, D.A.; Shatalin, I.V.; Karnup, S.V.
Morfologiia 127(3): 30-33
2005
ISSN/ISBN: 1026-3543 PMID: 16381308 Document Number: 586814
Using luminescent and absorption stains, specific for DNA (acridine orange, ethidium bromide), proteins (silver nitrate) and lipids (sudan III), the distribution of these substances was studied in the sections of rat olfactory bulbs, fixed by paraformaldehyde. DNA prevalence was found in glomerular cell layer as compared with the mitral one. Large amount of DNA was detected in granular cell layer, underlying the mitral one. The peculiarities of cellular layers of olfactory bulbs of 2-day-old rats were compared with those ones in 1-month-old animals. In rats of different ages, the differences were found in DNA content and distribution between layers, while no differences were detected in total protein and lipids. In 2-day-old rats glomerular underdevelopment was demonstrated.