Hemolytic potential of structurally related aniline halogenated hydroxylamines
Singh, H.; Purnell, E.T.
Journal of Environmental Pathology Toxicology and Oncology Official Organ of the International Society for Environmental Toxicology and Cancer 24(1): 67-76
2005
ISSN/ISBN: 0731-8898 PMID: 15715510 Document Number: 585946
This study was undertaken to investigate the hemolytic potential of several structurally related aniline halogenated phenylhydroxylamines based on their decreasing electronegativity. The compounds compared are phenylhydroxylamine (PHA) and para-fluoro-, para-bromo-, and para-iodo-phenylhydroxylamines. Red blood cells of male Sprague-Dawley rats were labeled,with radioactive chromium-51 and exposed to the test agent before being infused into the tail vein of isologous rats. The time course of blood radioactivity was monitored. The stability of some selected halogenated aniline analogs was also determined in blood. All four tested hydroxylamines produced dose-dependent reduction in the circulating labeled red blood cells indicating their destruction and loss. The most pronounced reduction was observed at doses from 175 to 250 muM. The dose of 100 muM appeared to be the threshold limit. The para-iodo-PHA was two times more toxic than para-fluoro-PHA in the destruction of red blood cells in rats.