Expression of glutathione S-transferase-pi and DNA topoisomerase Ii alpha and their implications in colorectal carcinoma
Ou, X.-l.; Sun, W.-h.; Xu, M.; Cao, D.-z.; Yu, Q.; Yu, T.; Sun, Y.-l.; Jiang, J.
Zhonghua Wei Chang Wai Ke Za Zhi 8(6): 524-527
2005
ISSN/ISBN: 1671-0274 PMID: 16299657 Document Number: 585109
To investigate the expression of GST-pi and Topo II-alpha, and their relationships with clinicopathological parameters in colorectal carcinoma. The expression of GST-pi and Topo II-alpha were detected by avidin-biotin-peroxide complex (ABC) method in tumor specimens, matched paratumor tissues from 60 cases with colorectal carcinoma and normal colonic tissues from 15 cases. The expression rates of GST-pi and Topo II-alpha were 90.0% and 86.7% respectively in tumor tissues, significantly higher than those in matched paratumor tissues and normal tissues (P< 0.01). The expressions of GST-pi and Topo II-alpha were associated with cellular differentiation, Dukes stage and lymph node metastasis (all P< 0.01), but not with tumor size and histological type (all P > 0.05). The expression level of GST-pi was significantly higher in poorly differentiated tumors than that in well differentiated tumors. The expression level of Topo II-alpha in well-differentiated tumors were stronger than that in poorly differentiated tumors. The detection of GST-pi and Topo II-alpha expressions may be helpful to judge the malignant behavior, metastasis and prognosis in human colorectal carcinoma.