Natural male contraceptive: phytochemical investigation and anti-spermatogenic activity of Pistia stratiotes Linn
Singh, K.; Dubey, B.Kumar.; Tripathi, A.C.; Singh, A.Pal.; Saraf, S.K.
Natural Product Research 28(16): 1313-1317
2014
ISSN/ISBN: 1478-6419 PMID: 24666370 DOI: 10.1080/14786419.2014.900772Document Number: 579566
This work is an attempt to explore the anti-spermatogenic activity of Pistia stratiotes and to investigate it as a male contraceptive. The prepared extracts were screened for the presence of alkaloids, glycosides, steroids, flavonoids, saponin and phenolic compounds. To assess the anti-spermatogenic activity, mice were orally administered with the various extracts of P. stratiotes (dose: 100 and 200 mg/kg body weight/day, for 45 days) and the most active, ethanolic extract was subjected to the isolation of phytoconstituent responsible for the activity. Diethyl ether fraction of ethanolic extract was taken to isolate a saponin, sitosterol-3-O-[2,4-di-O-acetyl-6-O-stearyl-β-D-glucopyranoside]. Anti-spermatogenic activity of the isolated saponin was evaluated at a dose of 50 mg/kg body weight/day, for 45 days. The treatment caused significant decrease (P < 0.01) in the weight of reproductive organs (testis, epididymis and seminal vesicle). The sperm count, sperm viability and serum testosterone levels were significantly lowered compared with that of the control group.