Identification of lymphogranuloma venereum-associated Chlamydia trachomatis serovars by fluorescence in situ hybridisation--a proof-of-principle analysis
Frickmann, H.; Essig, A.; Poppert, S.
Tropical Medicine and International Health Tm and Ih 19(4): 427-430
2014
ISSN/ISBN: 1365-3156 PMID: 24720755 DOI: 10.1111/tmi.12271Document Number: 579273
We describe a proof-of-principle evaluation of a fluorescence in situ hybridisation (FISH) procedure to identify Chlamydia trachomatis serovars L1-L3, the causative agents of lymphogranuloma venereum, in cell cultures based on newly designed DNA probes. Rapid and easy-to-perform FISH could facilitate the diagnosis of lymphogranuloma venereum without nucleic acid amplification or serotyping, but requires broader evaluation studies, for example, in tropical high-endemicity regions.
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