Identification of chlamydia and (or) chlamydia-like microorganisms by light microscopy confirmed by electron microscopy and fluorescent in situ hybridization
Zakharenko, L.P.; Rubtsov, N.B.; Karamysheva, T.V.; Riabchikova, E.I.; Bunkova, E.I.; Kolodina, N.N.; Nepomniashchaia, T.I.
Tsitologiia 45(12): 1221-1226
2003
ISSN/ISBN: 0041-3771 PMID: 15027355 Document Number: 554671
Using light microscopy, we have shown that chlamydia and/or chlamydia-like microorganisms are registered in 20-25% of the healthy part of human population, whereas in patients of the same age with gynecological problems these were found in 40-50%. Commonly, the infection was slightly manifested (less than 5% of cells are infected). These results were confirmed in four months but only in heavily infected patients. The light microscope data are confirmed by observations with electron microscopy, and by FISH hybridization of the total chlamydial DNA on cytological preparations with chlamydial inclusions. In some cases, microcolonies revealed by FISH hybridization occupied the majority of the cytoplasm volume. Occasionally, the DNA material was found on the nuclear surface. It seems likely that in heavily infected cells chlamydia are able to penetrate into the perinucular space.