Human mini-antibodies to orthopoxviruses
Morozova, V.V.; Tikunova, N.V.; Batanova, T.A.; Iun, T.E.; Bovshik, E.I.; Zhirakovskaia, E.V.; Voronina, V.V.; Bobko, D.I.; Belanov, E.F.; Il'ichev, A.A.; Sandakhchiev, L.S.
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk 2004(8): 22-27
2004
ISSN/ISBN: 0869-6047 PMID: 15455687 Document Number: 571171
A library of human scFv antibodies displayed on the surface of bacteriophages (MRC, Cambridge, England) was panned against the Elstree strain of vaccinia virus (VACV), which resulted in the phage repertoire enriched with clones positive to the strain. Individual clones from the repertoire were screened for binding, independently, to the vaccinia and ectromelia viruses; phage antibodies to the orthopoxviruses were selected. Ten unique antibodies were identified after their Vh- and VI-genes were sequenced. All selected antibodies were assayed by ELISA for binding to the vaccinia, cowpox and ectromelia viruses. Furthermore, all selected antibodies were assayed for binding with major alastrim strains of the live variola virus. According to the results, the above phage antibodies recognized genus-specific epitopes, some of which differed in their conformation.