The effect of growth factors on the proliferation of cultured porcine trabecular meshwork cells
Ando, A.; Kaneko, S.; Matsumura, M.
Nippon Ganka Gakkai Zasshi 108(9): 549-553
2004
ISSN/ISBN: 0029-0203 PMID: 15506488 Document Number: 570911
Purpose : We attempted to identify cell growth factors that cause a multiplication of trabecular meshwork (TM) cells. Methods : Porcine TM cells were cultured in Dulbecco's modified Eagle's medium/nutrient mixture F-12/Ham to which we added 1, 10, and 100 ng/ml of platelet-derived growth factor(PDGF), fibroblast growth factor 2(FGF2), insulin-like growth factor-1(IGF-1), vascular endothelial cell growth factor(VEGF), hepatocyte growth factor (HGF), or brain-derived neurotrophic factor(BDNF). We measured (3H) thymidine incorporation to evaluate the influence of the growth factors on TM cell proliferation. Results : (3H) thymidine incorporation into TM cells was promoted by 10 and 100 ng/ml of PDGF, IGF-1, and FGF 2 after 24 and 48 hours, whereas 1, 10, and 100 ng/ml of VEGF restrained cell proliferation after 48 hours. HGF and BDNF did not show any remarkable influence on TM cell proliferation. Conclusions : Our results suggest that PDGF, IGF-1, and FGF 2 may cause a drop in intraocular pressure followed by activation of a TM function, by multiplying TM cells.