Effect of Tujian decoction on protein kinase C activity in renal cortex in diabetic rat
Piao, Y-lin.; Liang, X-chun.; Yang, N.; Yin, D-hai.; Zuo, P-ping.
Zhongguo Zhong Yao Za Zhi 29(7): 685-689
2004
ISSN/ISBN: 1001-5302 PMID: 15503781 Document Number: 570215
Objective: To investigate influence of administration of Tujian decoction (Chinese herbal medicine) on protein kinase C (PKC) activity, renal function and structure in diabetic rat kidney. Method: Experimental diabetic nephropathy model was induced by nephrectomy combined with streptozotocin (STZ) injection in sprague-dawley rat. Tujian decoction (20 g.kg-1 .d-1) and Valsartan (20 mg . kg-1.d-1)were orally administrated respectively for 12 weeks. PKC activity was measured by (3H)phorbol 12, 13-dibutyrate ((3H)PDBu) binding assay. 24 h urine protein excretion (Upro) and renal pathological changes were observed. Result: In 12th week, diabetic nephrectomized rats developed proteinuria, glomerulosclerosis, increased membrane PKC activity (mPKC), decreased cytosol PKC (cPKC), and increased ratio of mPKC and cPKC (M/C). Administration of Tujian decoction or Valsartan led to a reduction in proteinuria, structural injury, mPKC and M/C, and a recovery in cPKC. Conclusion: Tujian decoction possesses a renoprotective effect on diabetic nephrectomized rat, at least partially via the inhibition of PKC activation in renal cortex.