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Spectrophotometry on measuring the size of liposomes

Niu, G-qin.; Pan, J.; Lu, W-yue.

Yao Xue Xue Bao 38(7): 547-551

2003

ISSN/ISBN: 0513-4870
PMID: 14515804
Document Number: 564775
Aim: To establish a spectrophotometric method for measurement of the sizes of liposomes for evaluating physical stability of liposomes. Methods: The sterically stabilized liposomes (SLs) were prepared by ethanol injection method and extrusion method. The mean cumulant diameters (D) of the vesicles were determined by electron microscopy and dynamic light scattering. On the basis of Rayleigh-Gans-Debye theory, the absorbance at 436 nm per unit lipid concentration (A436 nm/Cp) was measured as a function of vesicle diameter. Results: log(A436 nm/Cp) was closely related to logD (r2gtoreq0.93, n=5). Conclusion: The absorbance of liposomes reflect their relative sizes and can be used to evaluate physical stability of liposomes.

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