Studies on organ phytomorphology of leaves from Rehmannia glutinosa
Xue, J.-p.; Wang, R.-y.; Zhang, A.-m.; Liu, J.
Zhongguo Zhong Yao Za Zhi 28(1): 21-24
2003
ISSN/ISBN: 1001-5302 PMID: 15015260 Document Number: 563372
To set up the fittest medium and optimum condition for leaves from Rehmannia glutinosa to form regenerated plantlets directly and to induce microtubers. Leaves of different phases obtained from the virus-free plantlets were incubated in some kinds of media on the back side. The fittest medium for the formation of regenerated plantlet was MS + 6-BA 2.0 mg.L-1 and the fittest medium for the induction of microtuber was MS + 6-BA 1.0 mg.L-1 + NAA 0.5 mg.L-1. The optimum incubation temperaue was 25 degrees C and the light length was (2,000-3,000 lx) 12 h.d-1.