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Studies on immobilization of polyphenoloxidase from tea leaves and mango peel

Prabha, T.N.; Ramakrishna, M.; Patwardhan, M.V.; Raghavendra Rao, M.R.

Indian Journal of Biochemistry and Biophysics 18(6): 402-405

1981

ISSN/ISBN: 0301-1208
PMID: 7333627
Document Number: 178169
Polyphenoloxidase (EC 1.14.18.1) preparations from mango peel and green tea leaves were immobilized on various supports such as polyacrylamide gel, DEAE-Sephadex, DEAE-cellulose, collagen, arylamine glass and alkylamine glass. Some of the properties such as optimum temperature and pH, Km, substrate specificity, thermal stability, storage stability and reusability were studied and compared with those of native enzyme. Adsorption on DEAE-Sephadex and entrapment in polyacrylamide gel are satisfactory for immobilizing polyphenoloxidase from mango peel and tea leaves.

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