Analysis of nuclear and mitochondrial genomes of transplastomic Salpiglossis sinuata plants obtained by transfer of transformed plastids from N. tabacum (+S. sinuata) cybrid
Sytnik, E.S.; Pariĭ, A.F.; Komarnitskiĭ, I.K.; Gleba, I.I.; Kuchuk, N.V.
Tsitologiia i Genetika 37(5): 3-8
2003
ISSN/ISBN: 0564-3783 PMID: 14650322 Document Number: 562223
New model system of plastid transformation has been proposed using a wild representative of Solanaceae family--S. sinuata. Earlier obtained cybrid plants N. tabacum (+ S. sinuata) were used for transformation experiments by PEG treatment of protoplasts with aadA gene that confers resistance to spectinomycin. Transformed S. sinuata plastome was transferred from N. tabacum (+ S. sinuata) cybrid to S. sinuata wild type plants by somatic hybridization. Molecular analysis of nuclear and mitochondrial DNA has been performed.