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Arrangement of the sense and terminating codons of the template in the A-segment of human ribosomes from photocrosslinking data withe oligonucleotide derivatives

Stiazhkina, V.A.; Molotkov, M.V.; Demeshkina, N.A.; Bulygin, K.N.; Graĭfer, D.M.; Meshchaninova, M.I.; Repkova, M.N.; Ven'iaminova, A.G.; Karpova, G.G.

Molekuliarnaia Biologiia 37(6): 1019-1026

2003

ISSN/ISBN: 0026-8984
PMID: 14714497
Document Number: 559909
Oligoribonucleotide derivatives containing Phe codon UUC along with a 3'-flanking sense codon or stop codon carrying a perfluoroarylazido group at G or U were used to study the position of each nucleotide of the latter codon relative to the 18S rRNA in the A site of the 80S ribosome. To place the modified sense or stop codon in the A site, UCC-recognizing tRNA(Phe) was bound in the P site. Regardless of the position in the sense or stop codon, the modified nucleotide crosslinked with invariant dinucleotide A1823/A1824 or nucleotide A1825 in helix 44 close to the 3' end of the 18S rRNA. Located in the second or third position of either codon, the modified G bound with invariant nucleotide G626, which is in the evolutionarily conserved 530 stem-loop segment. The results were collated with the X-ray structure of the bacterial ribosome, and the template codon was assumed to be similarly arranged relative to the small-subunit rRNA in various organisms.

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